The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text
that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques
while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization
of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and
Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and principles
demonstrated are widely applicable to other systems. This text will serve as an excellent aid in the establishment
or instruction of introductory courses in the biological sciences.
Key Features of this new edition
All exercises and appendixes have been updated
Includes new exercises on
Polymerase chain reaction
Beta-Galactosidase detection in yeast colonies
Western blotting
New procedures introduced for
Large-scale plasmid isolation
Yeast transformation
DNA quantitation
New appendixes added, one of which provides details on accessing biological information sites on the Internet
(World Wide Web)
Use of non-radioactive materials and easy access to microbial cultures
Laboratory exercises student tested for seven years